Biochemical Characterization of Streptococcus pneumoniae Penicillin-Binding Protein 2b and Its Implication in -Lactam Resistance

نویسندگان

  • Estelle Pagliero
  • Laurent Chesnel
  • Julie Hopkins
  • Jacques Croizé
  • Otto Dideberg
  • Thierry Vernet
  • Anne Marie Di Guilmi
چکیده

Biochemical Characterization of Streptococcus pneumoniae Penicillin-Binding Protein 2b and Its Implication in -Lactam Resistance Estelle Pagliero, Laurent Chesnel, Julie Hopkins, Jacques Croizé, Otto Dideberg, Thierry Vernet,* and Anne Marie Di Guilmi Laboratoire d’Ingénierie des Macromolécules, and Laboratoire de Cristallographie Macromoléculaire, Institut de Biologie Structurale Jean-Pierre Ebel (CEA-CNRS UMR 5075-UJF), 38027 Grenoble Cedex 1, and Service de Bactériologie et Virologie, Centre Hospitalier Universitaire, 38043 Grenoble Cedex 09, France

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Biochemical characterization of Streptococcus pneumoniae penicillin-binding protein 2b and its implication in beta-lactam resistance.

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Acquisition of five high-Mr penicillin-binding protein variants during transfer of high-level beta-lactam resistance from Streptococcus mitis to Streptococcus pneumoniae.

Penicillin-resistant isolates of Streptococcus pneumoniae generally contain mosaic genes encoding the low-affinity penicillin-binding proteins (PBPs) PBP2x, PBP2b, and PBP1a. We now present evidence that PBP2a and PBP1b also appear to be low-affinity variants and are encoded by distinct alleles in beta-lactam-resistant transformants of S. pneumoniae obtained with chromosomal donor DNA from a St...

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Identical Penicillin-Binding Domains in Penicillin-Binding Proteins of Streptococcus pneumoniae Clinical Isolates with Different Levels of -Lactam Resistance

We have sequenced the penicillin-binding domains of the complete repertoire of penicillin-binding proteins and MurM from 22 clinical isolates of Streptococcus pneumoniae that span a wide range of -lactam resistance levels. Evidence of mosaicism was found in the genes encoding PBP 1a, PBP 2b, PBP 2x, MurM, and, possibly, PBP 2a. Five isolates were found to have identical PBP and MurM sequences, ...

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تاریخ انتشار 2004